Name of the analyte:
A sample of unknown composition, presumably
Laxogenin (Laxogenin, 5α-Hydroxy-Laxogenin is a synthetic steroid).
Appearance of the drug:
powder in a green capsule.
Purity Definition: HPLC with Diode Array Detector (Agilent 1100
series, HPLC-DAD). Detection λ = 210 nm. Qualitative analysis: HPLC with tandem
quadrupole time-of-flight mass detector (Agilent 6530 Accurate-Mass Q-TOF).
Conditions for chromatological analysis: column: Thermo Gold aQ 150 × 2.1 mm 3 μm; mobile phase:
A: 100% H O/0.01% TFA (v/v), B: 10% H O/90% ACN/0.01% UV 210 nm, the input volume is 20μl. 2 2
HPLC-DAD analysis results:
Figure 1 - chromatogram of the analyzed sample "Laxogenin".
The chromatogram (Fig. 1) has a peak with a retention time of 18.21 minutes. and absorption spectrum in UV
the area (Fig. 2) corresponds to "Laxogenin."
Figure 2 - UV spectrum of the analyzed sample Laxogenin (wavelength range 190 - 400 nm).
& nbsp; The table shows the relative contents of the components of the sample. The content of "Laxogenin" in the analyzed sample 79, 75%.
Table 1 - Results of HPLC-DAD Chromatogram Integration.
HPLC-MC analysis results:
Figure 3 - mass spectrum of the analyzed sample "Laxogenin", RT = 18.21.
& nbsp; In the mass spectrum (Fig. 3), a peak of 445, 2906 ± 0.0008 m/z and the isotopic distribution correspond to a + single-charged ion [Laxogenin + H].
In a sample of unknown composition, the substance “Laxogenin” was identified. Sample purity not less than 79.75%.